Project Partners

Eric Ezan

Employeed in CEA – Institute of Biology and technology Saclay (Ibitecs); Engineer in Biological Engineering from the University of Compiegne.

Got PhD in Pharmacological Sciences at the University of Paris V and joined the CEA in 1991.

In 2000, became Director of the Laboratory for Drug Metabolism Studies and from 2010 to 2014 he was at the Head of the Unit of Biochemistry of Toxicology at the CEA Marcoule.

Since 2014 the Scientific Advisor for the CEA Transversal Program “Technologies for Health”.

He has co-author in more than 110 publications in peer-review international journals and is frequently invited for conferences in international meetings.

Participates to various teaching in French universities and pharmaceutical industries.

He belongs to the Scientific board of big French institutions such the Agence Nationale de Santé et du Médicament (equivalent to FDA in France) or to Ibisa, the National consortium of Technological platform for Health.

Fields of expertises are Mass spectrometry applied to plant, food and biological media, Biotechnology, Biological and Environmental monitoring, Immunoanalysis, Mass Spectrometry for biological contminants.

1. Durighello E, Bellanger L, Ezan E, Armengaud J. Proteogenomic biomarkers for identification of Francisella species and subspecies by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Anal Chem. 2014 Oct 7;86(19):9394-8.

2. Chenau J, Fenaille F, Caro V, Haustant M, Diancourt L, Klee SR, Junot C, Ezan E, Goossens PL, Becher F. Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches. Mol Cell Proteomics. 2014 Mar;13(3):716-32.

3. Chenau J, Fenaille F, Ezan E, Morel N, Lamourette P, Goossens PL, Becher F. Sensitive detection of Bacillus anthracis spores by immunocapture and liquid chromatography-tandem mass spectrometry. Anal Chem. 2011 Nov 15;83(22):8675-82.

4. Villiers F, Ducruix C, Hugouvieux V, Jarno N, Ezan E, Garin J, Junot C, Bourguignon J. Investigating the plant response to cadmium exposure by proteomic and metabolomic approaches. Proteomics. 2011 May;11(9):1650-63

5. Chabre H, Gouyon B, Huet A, Baron-Bodo V, Nony E, Hrabina M, Fenaille F, Lautrette A, Bonvalet M, Maillère B, Bordas-Le Floch V, Van Overtvelt L, Jain K, Ezan E, Batard T, Moingeon P. Molecular variability of group 1 and 5 grass pollen allergens between Pooideae species: implications for immunotherapy. Clin Exp Allergy. 2010 Mar;40(3):505-19.

6. Fenaille F, Nony E, Chabre H, Lautrette A, Couret MN, Batard T, Moingeon P, Ezan E. Mass spectrometric investigation of molecular variability of grass pollen group 1 allergens. J Proteome Res. 2009 Aug;8(8):4014-27.

7. Duriez E, Goossens PL, Becher F, Ezan E. Femtomolar detection of the anthrax edema factor in human and animal plasma. Anal Chem. 2009 Jul 15;81(14):5935-41.

8. Duriez E, Fenaille F, Tabet JC, Lamourette P, Hilaire D, Becher F, Ezan E. Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Proteome Res. 2008 Sep;7(9):4154-63.

9. Becher F, Duriez E, Volland H, Tabet JC, Ezan E. Detection of functional ricin by immunoaffinity and liquid chromatography-tandem mass spectrometry. Anal Chem. 2007 Jan 15;79(2):659-65.

François Fenaille

François Fenaille (40 years old) received his diploma degree in analytical chemistry (1998) from the University of Nantes (France) and his PhD degree in analytical chemistry and mass spectrometry in 2004 from Pierre & Marie Curie University (Paris, France). In this period, he studied the modifications of milk lipids and proteins by mass spectrometric methods.

In 2005, he joined the French Laboratory of Fractionation and Biotechnology in Les Ulis (France), where his main interest was to develop and implement mass spectrometric techniques for the identification and structural characterization of proteins and their post-translational modifications.

Since 2007, he has been working as a research scientist in the team metabolome, biomarkers and therapeutic proteins leaded by Christophe Junot.

His field of research is mainly focused on the development of innovative mass spectrometry-based methods to characterize and quantify proteins, such as therapeutic proteins, biomarkers and toxins.

François Fenaille authored or co-authored 45 peer reviewed papers and 1 book chapter.

1. Chenau J, Fenaille F, Caro V, Haustant M, Diancourt L, Klee SR, Junot C, Ezan E, Goossens PL, Becher F. Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches. Mol Cell Proteomics. 2014 Mar;13(3):716-32.

2. Chenau J, Fenaille F, Ezan E, Morel N, Lamourette P, Goossens PL, Becher F. Sensitive detection of Bacillus anthracis spores by immunocapture and liquid chromatography-tandem mass spectrometry. Anal Chem. 2011 Nov 15;83(22):8675-82.

3. Chabre H, Gouyon B, Huet A, Baron-Bodo V, Nony E, Hrabina M, Fenaille F, Lautrette A, Bonvalet M, Maillère B, Bordas-Le Floch V, Van Overtvelt L, Jain K, Ezan E, Batard T, Moingeon P. Molecular variability of group 1 and 5 grass pollen allergens between Pooideae species: implications for immunotherapy. Clin Exp Allergy. 2010 Mar;40(3):505-19.

4. Fenaille F, Nony E, Chabre H, Lautrette A, Couret MN, Batard T, Moingeon P, Ezan E. Mass spectrometric investigation of molecular variability of grass pollen group 1 allergens. J Proteome Res. 2009 Aug;8(8):4014-27.

5. Duriez E, Fenaille F, Tabet JC, Lamourette P, Hilaire D, Becher F, Ezan E. Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Proteome Res. 2008 Sep;7(9):4154-63.

Christophe Junot

C. Junot is a doctor of Pharmacy.

After having gained a PhD in Analytical Chemistry in 2000 (Univ. Pierre et Marie Curie, Paris 6), he joined GlaxoSmithKline laboratories and developed experience in the field of pharmacokinetics and metabolism applied to drug discovery for 2 years.

From 2002, he has been a group leader at the Life Science Division of CEA (Commissariat à l’Energie Atomique) where he develops mass spectrometry based analytical methodologies for metabolome analysis.

C. Junot has been appointed as head of the Laboratory for Drug Metabolism Studies since September 2010 and he is member of the board of the French Metabolomics and Fluxomics Network.

C. Junot is the deputy coordinator of the French MetaboHUB platform and also in charge of the coordination of analytical chemistry developments within this platform.

1. Chenau J, Fenaille F, Caro V, Haustant M, Diancourt L, Klee SR, Junot C, Ezan E, Goossens PL, Becher F. Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches. Mol Cell Proteomics. 2014 Mar;13(3):716-32.

2. Villiers F, Ducruix C, Hugouvieux V, Jarno N, Ezan E, Garin J, Junot C, Bourguignon J. Investigating the plant response to cadmium exposure by proteomic and metabolomic approaches. Proteomics. 2011 May;11(9):1650-63

3. Villiers F, Ducruix C, Hugouvieux V, Jarno N, Ezan E, Garin J, Junot C, Bourguignon J. Investigating the plant response to cadmium exposure by proteomic and metabolomic approaches. Proteomics. 2011 May;11(9):1650-63

4. Cotton J, Leroux F, Broudin S, Marie M, Corman B, Tabet JC, Ducruix C, Junot C. High-resolution mass spectrometry associated with data mining tools for the detection of pollutants and chemical characterization of honey samples. J Agric. Food Chem. 2014 Nov 19;62(46):11335-45.

Valérie Tanchou

PhD in Biochemistry and virology, Paris. She has worked since 2006 on biosecurity/biodefense programs and managed biodetection projects. She created a real-time qPCR platform for genetic detection of biothreat agents. She was the head of a laboratory of detection and characterization of biothreat agents. Currently, she is an expert for the Join Research Center and she assumes the position of European correspondent for the iBEB in the following area: responses to environmental stresses in life science, plant and microbial biotechnology, and bioenergy, detection of environmental biological contaminations and bioremediation. 1. Tanchou V. Review of methods for the rapid ifdentification of pathogens in water samples. European Commission, JRC, ERNCIP. 2014 under publication. https://erncip-project.jrc.ec.europa.eu/networks/tgs/water Gas F, Baus B, Pinto L, Compere C, Tanchou V, Quéméneur E. One step immunochromatographic assay for the rapid detection of Alexandrium minutum. Biosens Bioelectron. 2010 Jan 15;25(5):1235-9